STochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution image by combining precise localization information for individual fluorophores in complex fluorescent microscope specimens. The N-STORM takes advantage of Nikon’s powerful Ti2-E inverted microscope and applies high-precision multi-color localization and three-dimensional (xyz) reconstruction to enable super-resolution imaging with tenfold the resolution of conventional light microscopes (down to 20 nm in xy). This powerful technology enables visualization of molecular localizations and arrangements at the nanoscale, opening new worlds of scientific understanding.